Menschen stellaris biografie

There are two main approaches being evaluated for submacular RPE transplantation-the injection of an RPE suspension and the transplantation of a monolayer RPE graft. A detailed comparison between the two methods is beyond the scope of this manuscript. However, the transplantation of a monolayer RPE graft may be advantageous as the RPE cells are more organized in a monolayer than in a suspension. RPE cells in the graft are organized in a confluent monolayer, which resembles the organization of the physiological RPE cell layer and enables the transplanted RPE cells to perform their physiological functions. This enables more precise dosing parameters compared to cell suspensions, which is highly relevant for regulatory work and industrial scale-up.

Delivery of the RPE patch graft into the subretinal space requires careful manipulation of the macula and accurate insertion of the graft in the subretinal space. Technological advances in microsurgery, such as miOCT, and a better understanding of intraoperative retinal tissue dynamics have reduced the learning curve of this procedure. In this discussion, the rationales of the following aspects will be explained: i) pre-operative plasminogen injection; ii) the use of intraoperative miOCT; iii) the use of a custom 41 G dual-bore cannul